In this experiment we used the Sanger Method of sequencing DNA. Or in other words the older version of sequencing DNA, considering that since this time we have advanced in technology and sequencing DNA has become easier and faster to be done. In the Sanger Method the letters are written down in threes in the order that they occur in the sequence. The groups of threes are placed and separated, then the selection of the protein begins that corresponds with the groups pattern. Abby was the first patient and she had only one letter difference, thus meaning she had a point mutation or a single base change. Bob was the next patient, he also had one difference in his DNA sequence. However with the type of protein sequence he acquired his sequence stopped short which lead to him having a truncation mutation. The last patient was Carol she had the most DNA sequencing differences from the normal sequence. Carol had a frameshift mutation. A frameshift mutation is where there is a gain or loss of a letter that throws the rest of the sequence off from its normal pattern. So although we all have DNA and it has its own sequencing pattern there are plenty of mutations that can occur and cause the sequence to mess up or end overall.
That is how you read a sequence and the three things that can go wron
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